FTO Knockout cell line (Hep G2)

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the FTO gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, FTO gene knockout FTO Knockout cell line (Hep G2) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

Catalog#

YKO-HT1670

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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FTO Knockout cell line (Hep G2)

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